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anti mouse notch2 pabs  (R&D Systems)


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    R&D Systems anti mouse notch2 pabs
    Adhesion of MCs to Notch ligand-transduced OP9 stromal cells was not inhibited by the insufficiency of Kit in MCs. (A) WT MCs were cultured on each OP9 stromal cell with 5 µg ml−1 of anti-IL-7Rα mAb (Ctrl mAb) or anti-Kit mAb (ACK2). The percentages of adherent MCs at each incubation time (5, 15, 30, 60 and 120 min) are shown. Data are the mean ± SE of triplicate cultures (*P < 0.05 between Ctrl mAb and anti-Kit mAb at each time point, two-tailed Welch’s t-test). (B) Surface expression of Kit and FcɛRIα on MCs-Kit +/+ and -Kit W-sh/W-sh . (C) Percentages of adherent MCs-Kit +/+ and -Kit W-sh/W-sh on each OP9 stromal cell at 60 min of the adhesion assay are shown (mean ± SE of triplicate cultures; *P < 0.05, two-tailed Welch’s t-test). (D) Surface expression of Notch1 and <t>Notch2</t> on MCs-Kit +/+ and -Kit W-sh/W-sh , and Kit+ peritoneal MCs (see Supplementary Figure 2). (E) The expression of Kit and Fcer1a in the mixed lysates of adherent MCs-Kit +/+ or -Kit W-sh/W-sh and each OP9 stromal cell after 60 min of the adhesion assay was analyzed by RT–PCR. RT–PCR with total RNAs from each MC alone before the adhesion assay was designated as ‘Pre’. (F) The expression of Kit, Fcer1a and Kitl in each OP9 stromal cell was analyzed by RT–PCR. (E, F) Original gel sources and negative controls are in Supplementary Figure 1.
    Anti Mouse Notch2 Pabs, supplied by R&D Systems, used in various techniques. Bioz Stars score: 90/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+mouse+notch2+pabs/Mouse+Notch-2+Antibody/pmc08562337-86-20-23
    Average 90 stars, based on 3 article reviews
    anti mouse notch2 pabs - by Bioz Stars, 2026-10
    90/100 stars

    Images

    1) Product Images from "Kit-independent mast cell adhesion mediated by Notch"

    Article Title: Kit-independent mast cell adhesion mediated by Notch

    Journal: International Immunology

    doi: 10.1093/intimm/dxy067

    Adhesion of MCs to Notch ligand-transduced OP9 stromal cells was not inhibited by the insufficiency of Kit in MCs. (A) WT MCs were cultured on each OP9 stromal cell with 5 µg ml−1 of anti-IL-7Rα mAb (Ctrl mAb) or anti-Kit mAb (ACK2). The percentages of adherent MCs at each incubation time (5, 15, 30, 60 and 120 min) are shown. Data are the mean ± SE of triplicate cultures (*P < 0.05 between Ctrl mAb and anti-Kit mAb at each time point, two-tailed Welch’s t-test). (B) Surface expression of Kit and FcɛRIα on MCs-Kit +/+ and -Kit W-sh/W-sh . (C) Percentages of adherent MCs-Kit +/+ and -Kit W-sh/W-sh on each OP9 stromal cell at 60 min of the adhesion assay are shown (mean ± SE of triplicate cultures; *P < 0.05, two-tailed Welch’s t-test). (D) Surface expression of Notch1 and Notch2 on MCs-Kit +/+ and -Kit W-sh/W-sh , and Kit+ peritoneal MCs (see Supplementary Figure 2). (E) The expression of Kit and Fcer1a in the mixed lysates of adherent MCs-Kit +/+ or -Kit W-sh/W-sh and each OP9 stromal cell after 60 min of the adhesion assay was analyzed by RT–PCR. RT–PCR with total RNAs from each MC alone before the adhesion assay was designated as ‘Pre’. (F) The expression of Kit, Fcer1a and Kitl in each OP9 stromal cell was analyzed by RT–PCR. (E, F) Original gel sources and negative controls are in Supplementary Figure 1.
    Figure Legend Snippet: Adhesion of MCs to Notch ligand-transduced OP9 stromal cells was not inhibited by the insufficiency of Kit in MCs. (A) WT MCs were cultured on each OP9 stromal cell with 5 µg ml−1 of anti-IL-7Rα mAb (Ctrl mAb) or anti-Kit mAb (ACK2). The percentages of adherent MCs at each incubation time (5, 15, 30, 60 and 120 min) are shown. Data are the mean ± SE of triplicate cultures (*P < 0.05 between Ctrl mAb and anti-Kit mAb at each time point, two-tailed Welch’s t-test). (B) Surface expression of Kit and FcɛRIα on MCs-Kit +/+ and -Kit W-sh/W-sh . (C) Percentages of adherent MCs-Kit +/+ and -Kit W-sh/W-sh on each OP9 stromal cell at 60 min of the adhesion assay are shown (mean ± SE of triplicate cultures; *P < 0.05, two-tailed Welch’s t-test). (D) Surface expression of Notch1 and Notch2 on MCs-Kit +/+ and -Kit W-sh/W-sh , and Kit+ peritoneal MCs (see Supplementary Figure 2). (E) The expression of Kit and Fcer1a in the mixed lysates of adherent MCs-Kit +/+ or -Kit W-sh/W-sh and each OP9 stromal cell after 60 min of the adhesion assay was analyzed by RT–PCR. RT–PCR with total RNAs from each MC alone before the adhesion assay was designated as ‘Pre’. (F) The expression of Kit, Fcer1a and Kitl in each OP9 stromal cell was analyzed by RT–PCR. (E, F) Original gel sources and negative controls are in Supplementary Figure 1.

    Techniques Used: Cell Culture, Incubation, Two Tailed Test, Expressing, Cell Adhesion Assay, Reverse Transcription Polymerase Chain Reaction

    Spreading of adherent MCs-Kit W-sh/W-sh on Notch ligand-transduced OP9 stromal cells was dependent on the Notch receptor–ligand interaction, but independent of γ-secretase-mediated Notch signaling. (A) Adhesion assays (60 min) for MCs-Kit W-sh/W-sh on each OP9 cell with control pAb (20 µg ml−1) or anti-Notch1 pAb plus anti-Notch2 pAb (10 µg ml−1 each). Cultures contained 76.9 µM NaN3, which had no effect on the adhesion of MCs. (B) MCs-Kit W-sh/W-sh were pre-treated with the γ-secretase inhibitor DAPT (50 µM) or its solvent DMSO (0.5% vol/vol) for 30 min, and the adhesion assay (60 min) was performed with the reagents. (C) MCs-Kit +/+ were pre-treated with an anti-Kit mAb (ACK2, 10 µg ml−1) plus DAPT (30 µM) or DMSO (0.3% vol/vol) for 10 min, and the adhesion assay (60 min) was performed with the reagents. Data are the mean ± SE of triplicate cultures. Asterisks denote the significantly different responses of each adhesion state from control treatments (*P < 0.05, two-tailed Welch’s t-test).
    Figure Legend Snippet: Spreading of adherent MCs-Kit W-sh/W-sh on Notch ligand-transduced OP9 stromal cells was dependent on the Notch receptor–ligand interaction, but independent of γ-secretase-mediated Notch signaling. (A) Adhesion assays (60 min) for MCs-Kit W-sh/W-sh on each OP9 cell with control pAb (20 µg ml−1) or anti-Notch1 pAb plus anti-Notch2 pAb (10 µg ml−1 each). Cultures contained 76.9 µM NaN3, which had no effect on the adhesion of MCs. (B) MCs-Kit W-sh/W-sh were pre-treated with the γ-secretase inhibitor DAPT (50 µM) or its solvent DMSO (0.5% vol/vol) for 30 min, and the adhesion assay (60 min) was performed with the reagents. (C) MCs-Kit +/+ were pre-treated with an anti-Kit mAb (ACK2, 10 µg ml−1) plus DAPT (30 µM) or DMSO (0.3% vol/vol) for 10 min, and the adhesion assay (60 min) was performed with the reagents. Data are the mean ± SE of triplicate cultures. Asterisks denote the significantly different responses of each adhesion state from control treatments (*P < 0.05, two-tailed Welch’s t-test).

    Techniques Used: Control, Solvent, Cell Adhesion Assay, Two Tailed Test

    Related Articles

    Staining:

    Article Title: Kit-independent mast cell adhesion mediated by Notch
    Article Snippet: Cells were initially incubated with 33% rabbit serum (Gibco) with anti-CD16/32 (2.4G2, Tonbo Biosciences) for blocking, and then stained with fluorescein-labeled mAbs and PI. .. Regarding the detection of Notch receptors in , cells were stained with fluorescein-labeled mAbs and sheep control, anti-mouse Notch1 or anti-mouse Notch2 pAbs (R&D Systems), and then with rabbit anti-sheep IgG-biotin (Vector Laboratories) and streptavidin-PE (SouthernBiotech). ..

    Control:

    Article Title: Kit-independent mast cell adhesion mediated by Notch
    Article Snippet: Cells were initially incubated with 33% rabbit serum (Gibco) with anti-CD16/32 (2.4G2, Tonbo Biosciences) for blocking, and then stained with fluorescein-labeled mAbs and PI. .. Regarding the detection of Notch receptors in , cells were stained with fluorescein-labeled mAbs and sheep control, anti-mouse Notch1 or anti-mouse Notch2 pAbs (R&D Systems), and then with rabbit anti-sheep IgG-biotin (Vector Laboratories) and streptavidin-PE (SouthernBiotech). ..



    Similar Products

    anti mouse notch2 pabs  (R&D Systems)
    90
    R&D Systems anti mouse notch2 pabs
    Adhesion of MCs to Notch ligand-transduced OP9 stromal cells was not inhibited by the insufficiency of Kit in MCs. (A) WT MCs were cultured on each OP9 stromal cell with 5 µg ml−1 of anti-IL-7Rα mAb (Ctrl mAb) or anti-Kit mAb (ACK2). The percentages of adherent MCs at each incubation time (5, 15, 30, 60 and 120 min) are shown. Data are the mean ± SE of triplicate cultures (*P < 0.05 between Ctrl mAb and anti-Kit mAb at each time point, two-tailed Welch’s t-test). (B) Surface expression of Kit and FcɛRIα on MCs-Kit +/+ and -Kit W-sh/W-sh . (C) Percentages of adherent MCs-Kit +/+ and -Kit W-sh/W-sh on each OP9 stromal cell at 60 min of the adhesion assay are shown (mean ± SE of triplicate cultures; *P < 0.05, two-tailed Welch’s t-test). (D) Surface expression of Notch1 and <t>Notch2</t> on MCs-Kit +/+ and -Kit W-sh/W-sh , and Kit+ peritoneal MCs (see Supplementary Figure 2). (E) The expression of Kit and Fcer1a in the mixed lysates of adherent MCs-Kit +/+ or -Kit W-sh/W-sh and each OP9 stromal cell after 60 min of the adhesion assay was analyzed by RT–PCR. RT–PCR with total RNAs from each MC alone before the adhesion assay was designated as ‘Pre’. (F) The expression of Kit, Fcer1a and Kitl in each OP9 stromal cell was analyzed by RT–PCR. (E, F) Original gel sources and negative controls are in Supplementary Figure 1.
    Anti Mouse Notch2 Pabs, supplied by R&D Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+mouse+notch2+pabs/Mouse+Notch-2+Antibody/pmc08562337-86-20-23
    Average 90 stars, based on 1 article reviews
    anti mouse notch2 pabs - by Bioz Stars, 2026-10
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    Adhesion of MCs to Notch ligand-transduced OP9 stromal cells was not inhibited by the insufficiency of Kit in MCs. (A) WT MCs were cultured on each OP9 stromal cell with 5 µg ml−1 of anti-IL-7Rα mAb (Ctrl mAb) or anti-Kit mAb (ACK2). The percentages of adherent MCs at each incubation time (5, 15, 30, 60 and 120 min) are shown. Data are the mean ± SE of triplicate cultures (*P < 0.05 between Ctrl mAb and anti-Kit mAb at each time point, two-tailed Welch’s t-test). (B) Surface expression of Kit and FcɛRIα on MCs-Kit +/+ and -Kit W-sh/W-sh . (C) Percentages of adherent MCs-Kit +/+ and -Kit W-sh/W-sh on each OP9 stromal cell at 60 min of the adhesion assay are shown (mean ± SE of triplicate cultures; *P < 0.05, two-tailed Welch’s t-test). (D) Surface expression of Notch1 and Notch2 on MCs-Kit +/+ and -Kit W-sh/W-sh , and Kit+ peritoneal MCs (see Supplementary Figure 2). (E) The expression of Kit and Fcer1a in the mixed lysates of adherent MCs-Kit +/+ or -Kit W-sh/W-sh and each OP9 stromal cell after 60 min of the adhesion assay was analyzed by RT–PCR. RT–PCR with total RNAs from each MC alone before the adhesion assay was designated as ‘Pre’. (F) The expression of Kit, Fcer1a and Kitl in each OP9 stromal cell was analyzed by RT–PCR. (E, F) Original gel sources and negative controls are in Supplementary Figure 1.

    Journal: International Immunology

    Article Title: Kit-independent mast cell adhesion mediated by Notch

    doi: 10.1093/intimm/dxy067

    Figure Lengend Snippet: Adhesion of MCs to Notch ligand-transduced OP9 stromal cells was not inhibited by the insufficiency of Kit in MCs. (A) WT MCs were cultured on each OP9 stromal cell with 5 µg ml−1 of anti-IL-7Rα mAb (Ctrl mAb) or anti-Kit mAb (ACK2). The percentages of adherent MCs at each incubation time (5, 15, 30, 60 and 120 min) are shown. Data are the mean ± SE of triplicate cultures (*P < 0.05 between Ctrl mAb and anti-Kit mAb at each time point, two-tailed Welch’s t-test). (B) Surface expression of Kit and FcɛRIα on MCs-Kit +/+ and -Kit W-sh/W-sh . (C) Percentages of adherent MCs-Kit +/+ and -Kit W-sh/W-sh on each OP9 stromal cell at 60 min of the adhesion assay are shown (mean ± SE of triplicate cultures; *P < 0.05, two-tailed Welch’s t-test). (D) Surface expression of Notch1 and Notch2 on MCs-Kit +/+ and -Kit W-sh/W-sh , and Kit+ peritoneal MCs (see Supplementary Figure 2). (E) The expression of Kit and Fcer1a in the mixed lysates of adherent MCs-Kit +/+ or -Kit W-sh/W-sh and each OP9 stromal cell after 60 min of the adhesion assay was analyzed by RT–PCR. RT–PCR with total RNAs from each MC alone before the adhesion assay was designated as ‘Pre’. (F) The expression of Kit, Fcer1a and Kitl in each OP9 stromal cell was analyzed by RT–PCR. (E, F) Original gel sources and negative controls are in Supplementary Figure 1.

    Article Snippet: Regarding the detection of Notch receptors in , cells were stained with fluorescein-labeled mAbs and sheep control, anti-mouse Notch1 or anti-mouse Notch2 pAbs (R&D Systems), and then with rabbit anti-sheep IgG-biotin (Vector Laboratories) and streptavidin-PE (SouthernBiotech).

    Techniques: Cell Culture, Incubation, Two Tailed Test, Expressing, Cell Adhesion Assay, Reverse Transcription Polymerase Chain Reaction

    Spreading of adherent MCs-Kit W-sh/W-sh on Notch ligand-transduced OP9 stromal cells was dependent on the Notch receptor–ligand interaction, but independent of γ-secretase-mediated Notch signaling. (A) Adhesion assays (60 min) for MCs-Kit W-sh/W-sh on each OP9 cell with control pAb (20 µg ml−1) or anti-Notch1 pAb plus anti-Notch2 pAb (10 µg ml−1 each). Cultures contained 76.9 µM NaN3, which had no effect on the adhesion of MCs. (B) MCs-Kit W-sh/W-sh were pre-treated with the γ-secretase inhibitor DAPT (50 µM) or its solvent DMSO (0.5% vol/vol) for 30 min, and the adhesion assay (60 min) was performed with the reagents. (C) MCs-Kit +/+ were pre-treated with an anti-Kit mAb (ACK2, 10 µg ml−1) plus DAPT (30 µM) or DMSO (0.3% vol/vol) for 10 min, and the adhesion assay (60 min) was performed with the reagents. Data are the mean ± SE of triplicate cultures. Asterisks denote the significantly different responses of each adhesion state from control treatments (*P < 0.05, two-tailed Welch’s t-test).

    Journal: International Immunology

    Article Title: Kit-independent mast cell adhesion mediated by Notch

    doi: 10.1093/intimm/dxy067

    Figure Lengend Snippet: Spreading of adherent MCs-Kit W-sh/W-sh on Notch ligand-transduced OP9 stromal cells was dependent on the Notch receptor–ligand interaction, but independent of γ-secretase-mediated Notch signaling. (A) Adhesion assays (60 min) for MCs-Kit W-sh/W-sh on each OP9 cell with control pAb (20 µg ml−1) or anti-Notch1 pAb plus anti-Notch2 pAb (10 µg ml−1 each). Cultures contained 76.9 µM NaN3, which had no effect on the adhesion of MCs. (B) MCs-Kit W-sh/W-sh were pre-treated with the γ-secretase inhibitor DAPT (50 µM) or its solvent DMSO (0.5% vol/vol) for 30 min, and the adhesion assay (60 min) was performed with the reagents. (C) MCs-Kit +/+ were pre-treated with an anti-Kit mAb (ACK2, 10 µg ml−1) plus DAPT (30 µM) or DMSO (0.3% vol/vol) for 10 min, and the adhesion assay (60 min) was performed with the reagents. Data are the mean ± SE of triplicate cultures. Asterisks denote the significantly different responses of each adhesion state from control treatments (*P < 0.05, two-tailed Welch’s t-test).

    Article Snippet: Regarding the detection of Notch receptors in , cells were stained with fluorescein-labeled mAbs and sheep control, anti-mouse Notch1 or anti-mouse Notch2 pAbs (R&D Systems), and then with rabbit anti-sheep IgG-biotin (Vector Laboratories) and streptavidin-PE (SouthernBiotech).

    Techniques: Control, Solvent, Cell Adhesion Assay, Two Tailed Test